Inhibition of blood cholinesterases by nerve agents in vitro
نویسندگان
چکیده
منابع مشابه
Inhibition of cholinesterases by fluoride in vitro.
1. Series of colorimetric dynamic assays allowed the study of the inhibition of cholinesterases by F(-) ions in vitro, by using, as sources of enzyme, whole human blood, human serum, homogenized rat brain and two preparations of red blood cells (human and bovine) whose enzymic purity was ascertained. 2. The first evidence of inhibition of human serum pseudocholinesterase by fluoride was noticed...
متن کاملIn vitro inhibition of cholinesterases by carbamates--a kinetic study.
Kinetics and mechanism of in vitro hydrolyses of acetylcholine and acetylthiocholine by carbamates were studied in a batch reactor at 25 degrees C, pH 8, and ionic strength of 0.11 M. Every hydrolysis was monitored by 3-4 independent methods. All studied hydrolyses can be described by the model of competitive inhibition with an irreversible step (k3). A table of obtained average values of rate ...
متن کاملThe inhibition of cholinesterases by pancuronium.
Pancuronium causes a powerful and highly selective inhibition of human serum cholinesterase in vitro. The inhibition was studied in serum from 14 individuals of both sexes (5-60 years of age) with normal reactions to suxamethonium. Pancuronium, in a concentration of 2.3 x 10(-7) M, caused a 50% inhibition of the enzymatic hydrolysis of acetylcholine, when this substrate was present in a concen...
متن کاملInhibition of blood and tissue cholinesterases by soman in guinea pigs in vivo
Guinea pigs were intoxicated intramuscularly with different doses of soman, and cholinesterase activities were determined in the blood, diaphragm and parts of the brain – the pontomedullar area, the frontal cortex and the basal ganglia. The time course of poisoning following low doses (1,3, 5 μg/kg) and a dose equal to 1×LD50 (28.5 μg/kg) were studied. The dose having a negligible effect on cho...
متن کامل[Cholinesterases: structure, role, and inhibition].
Enzymes acetylcholinesterase (AChE; E.C. 3.1.1.7) and butyrylcholinesterase (BChE; E.C. 3.1.1.8) have intensively been investigated in biomedicine and toxicology due to important role in organisms. Even if structurally homologous, they differ in catalytic activity, specificity, for substrates, and selectivity in binding to many ligands. This paper compiles the results of research on cholinester...
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ژورنال
عنوان ژورنال: Journal of Applied Biomedicine
سال: 2009
ISSN: 1214-021X,1214-0287
DOI: 10.32725/jab.2009.022